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PCR |
| Name: |
CerQuant |
Type: |
Real-Time PCR |
System: |
Quantitative |
| Genetic Element: |
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Ampl. Length: |
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Ampl. Position: |
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Sequence Size [bp]: |
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| AnnTemp [°C]: |
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Taxonomy: |
Cereals: Oat-Barley-Rye-Wheat |
Temp. Time Program: |
1 X<br>95°C 5 min<br>95°C 10 sec ; acquisition mode "none"<br>45 X<br>57°C 60 sec ; acquisition mode "single"<br>40°C 30 sec |
| Device: |
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Reagents: |
Reagents Vol/PCR<br>(μL)<br>H2O 7.15<br>PCR Buffer Takara (10X) 2<br>MgCl2 (25mM) 1.2<br>BSA (0.02μg/μL) 2<br>dNTP (2.5mM) 2<br>Primer Cer1 F (20μM) 0.9<br>Primer Cer2R (20μM) 0.9<br>Probe fluoCer1 (20μM) 0.8<br>Probe redCer1-APD (20μM) 0.8<br>TaqPol Takara (5U/μl) 0.25<br>Volume total 18 μL<br>DNA : 2 μL |
nPrimers: |
4 |
Organisation: |
Eurofins |
| PCR Description: |
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Sequence: |
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| LOD [copy]: |
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LOQ [copy]: |
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Efficiency [%]: |
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| Remarks: |
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Food/Product |
| Food Name: |
Generic Image: |
Product Type: |
Food Source: |
Product Website: |
| Wheat |  | Cereals and cereal products [A0457] | | |
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
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Cer1F |
Sense |
AAT CCT gAg CCA AAT CCg Tg |
20 |
66.9 |
58.1 |
60 |
50.00 |
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Cer2R |
Antisense |
CAT gTA gAA Tgg gAC TCT CTC TTT g |
25 |
64.8 |
56.0 |
72 |
44.00 |
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fluoCER1 |
Marker/Probe |
5&q uot ; T AAC TCg ATT CgT TAg AAC AgC TTC CAT TgA gTC TCT -- FL |
37 |
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redCER1_APD |
Marker/Probe |
5&q uot ; L C R ed6 40 - C ACC TAT CCT TTT CCT TTg TAT TCT AgT TCg AgA PH |
34 |
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