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PCR |
| Name: |
ETH225(D9S1) |
Type: |
Microsatellite |
System: |
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| Genetic Element: |
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Ampl. Length: |
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Ampl. Position: |
C9: 131-159 |
Sequence Size [bp]: |
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| AnnTemp [°C]: |
55-65 |
Taxonomy: |
Cattle (Beef (general)) |
Temp. Time Program: |
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| Device: |
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Reagents: |
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nPrimers: |
2 |
Organisation: |
UU-FDG |
| PCR Description: |
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Sequence: |
Z14043 |
| LOD [copy]: |
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LOQ [copy]: |
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Efficiency [%]: |
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| Remarks: |
Markers are also used in the Stockmarket kit from ABI for routine parentage testing |
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
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ETH225-f |
Sense |
GAT CAC CTT GCC ACT ATT TCC T |
22 |
55-65 |
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ETH225-r |
Antisense |
ACA TGA CAG CCA GCT GCT ACT |
21 |
55-65 |
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| NCBI-Acc: |
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Sequence Description: |
B.taurus microsatellite (ETH225). |
| BaseCount: |
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| DNA-Sequence: |
1 gatcaccttg ccactatttc ctccaacata tgtgtgtgcg tgcacacaca cac |
AminoAcid-Sequence: |
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