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PCR |
| Name: |
act |
Type: |
Real-Time PCR |
System: |
Quantitative |
| Genetic Element: |
Actin |
Ampl. Length: |
103 |
Ampl. Position: |
|
Sequence Size [bp]: |
|
| AnnTemp [°C]: |
|
Taxonomy: |
Plants |
Temp. Time Program: |
Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60 |
| Device: |
|
Reagents: |
|
nPrimers: |
3 |
Organisation: |
BfR |
| PCR Description: |
|
Sequence: |
AY372368 |
| LOD [copy]: |
1 |
LOQ [copy]: |
10 |
Efficiency [%]: |
107 |
| Remarks: |
|
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
act-f |
Sense |
CAA gCA gCA TgA AgA TCA Agg T |
22 |
66.3 |
57.5 |
64 |
45.45 |
|
|
act-r |
Antisense |
CAC ATC TgT Tgg AAA gTg CTg Ag |
23 |
66.8 |
58.1 |
68 |
47.83 |
|
|
act-probe |
Marker/Probe |
FAM - CCT CCA ATC CAg ACA CTg TAC TTT CTC TC - T AMR A |
29 |
70.9 |
62.2 |
86 |
48.28 |
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| NCBI-Acc: |
|
Sequence Description: |
Trifolium pratense actin mRNA, complete cds. |
| BaseCount: |
|
| DNA-Sequence: |
|
AminoAcid-Sequence: |
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