|
You are here: Core Forms > Search PCR > PCR
 First |  Previous | | Next  | Last  |
PCR |
| Name: |
Tri_act |
Type: |
Real-Time PCR |
System: |
Quantitative |
| Genetic Element: |
Actin |
Ampl. Length: |
191 |
Ampl. Position: |
|
Sequence Size [bp]: |
|
| AnnTemp [°C]: |
58 |
Taxonomy: |
Plants |
Temp. Time Program: |
Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60 |
| Device: |
|
Reagents: |
|
nPrimers: |
3 |
Organisation: |
BfR |
| PCR Description: |
|
Sequence: |
AY372368 |
| LOD [copy]: |
1 |
LOQ [copy]: |
10 |
Efficiency [%]: |
107 |
| Remarks: |
|
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
Tri_act-f |
Sense |
CTg gCC gTg ATC TAA CTg AAT CTT |
24 |
67.7 (PCR: 58) |
58.9 |
70 |
45.83 |
|
|
Tri_act-r |
Antisense |
ATC Agg AAg CTC ATA gTT TTT CTC AAT T |
28 |
66.9 (PCR: 58) |
58.2 |
74 |
32.14 |
|
|
Tri_act-probe |
Marker/Probe |
5Ž -FA M - AA GTT CTT GTT CAT AAT CCA CAG CAA CAT AGG CAA -( TAM RA) -3Å ½ |
35 |
PCR: 58 |
|
|
|
|
|
| NCBI-Acc: |
|
Sequence Description: |
Trifolium pratense actin mRNA, complete cds. |
| BaseCount: |
|
| DNA-Sequence: |
|
AminoAcid-Sequence: |
|
|
You are here: Core Forms > Search PCR > PCR
|
Overview