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PCR |
| Name: |
Euc_LFY |
Type: |
Real-Time PCR |
System: |
Quantitative |
| Genetic Element: |
LEAFY/FLORICAULA (LFY1) |
Ampl. Length: |
96 |
Ampl. Position: |
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Sequence Size [bp]: |
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| AnnTemp [°C]: |
60 |
Taxonomy: |
Eucalyptus grandis |
Temp. Time Program: |
Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60<br> |
| Device: |
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Reagents: |
|
nPrimers: |
3 |
Organisation: |
BfR |
| PCR Description: |
|
Sequence: |
AY640313 |
| LOD [copy]: |
5 |
LOQ [copy]: |
50 |
Efficiency [%]: |
93 |
| Remarks: |
|
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
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Euc_LFY-f |
Sense |
Agg AgC AAg Tgg TgC AgC A |
19 |
68.9 (PCR: 60) |
60.2 |
60 |
57.89 |
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Euc_LFY-r |
Antisense |
TCC TCT gTT gTT ggg CgC |
18 |
68.5 (PCR: 60) |
59.8 |
58 |
61.11 |
|
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Euc_LFY-probe |
Marker/Probe |
5Ž -FA M)- TC AgA AAA ggA TCA gCT ggg CAg gg -(T AMR A)- 3Ž |
25 |
72.1 (PCR: 60) |
67.8 |
78 |
56.00 |
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| NCBI-Acc: |
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Sequence Description: |
Eucalyptus grandis LEAFY/FLORICAULA (LFY1) gene, complete cds. |
| BaseCount: |
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| DNA-Sequence: |
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AminoAcid-Sequence: |
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