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PCR |
| Name: |
B.aphidicola |
Type: |
Real-Time PCR |
System: |
Qualitative |
| Genetic Element: |
Plasmid pTrp |
Ampl. Length: |
64 |
Ampl. Position: |
1165-1102 |
Sequence Size [bp]: |
1506.Buchnera aphidicola 16S ribosomal RNA gene, |
| AnnTemp [°C]: |
66-72 |
Taxonomy: |
Greenbug Bacteria |
Temp. Time Program: |
|
| Device: |
|
Reagents: |
|
nPrimers: |
3 |
Organisation: |
Biolytix |
| PCR Description: |
Plasmid-Genom pTrp enthalten Plasmide mit Genen für die Biosynthese von Trypthophan und Leucin.<br>Buchnera aphidicola 16S ribosomal RNA gene, complete sequence |
Sequence: |
Plasmid pTrp |
| LOD [copy]: |
|
LOQ [copy]: |
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Efficiency [%]: |
|
| Remarks: |
AmpliconLenght 64 or 69? |
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
B.aphi-F |
Sense |
TTA TAA CCg gCA gTC TCC TCT gA |
23 |
66.7 |
57.9 |
68.0 |
47.8 |
1165-1143 |
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B.aphi-R |
Antisense |
CgC AAC CCT TAT CCC CTg TT |
20 |
68.9 |
60.1 |
62.0 |
55.0 |
1221-1102 |
|
B.aphi-probe |
Marker/Probe |
TTC CCg gCC gAA CCg CTg |
18 |
72.1 |
68.0 |
62.0 |
72.2 |
1141-1124 |
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