|
You are here: Core Forms > Search PCR > PCR
 First |  Previous | | Next  | Last  |
PCR |
| Name: |
Til_nr |
Type: |
Real-Time PCR |
System: |
Quantitative |
| Genetic Element: |
nitrate reductase |
Ampl. Length: |
101 |
Ampl. Position: |
|
Sequence Size [bp]: |
|
| AnnTemp [°C]: |
58 |
Taxonomy: |
Lime, Broad-leaved (Tilia platyphyllos) |
Temp. Time Program: |
Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60<br> |
| Device: |
|
Reagents: |
|
nPrimers: |
3 |
Organisation: |
BfR |
| PCR Description: |
|
Sequence: |
AY138811 |
| LOD [copy]: |
25 |
LOQ [copy]: |
250 |
Efficiency [%]: |
98 |
| Remarks: |
|
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
Til_nr-f |
Sense |
ggg CAT CgA ACA TgA gCT TT |
20 |
67.0 (PCR: 58) |
58.3 |
60 |
50.00 |
|
|
Til_nr-r |
Antisense |
TTC AAC gAg TTT gCA Tgg gA |
20 |
66.6 (PCR: 58) |
57.8 |
58 |
45.00 |
|
|
Til_nr-probe |
Marker/Probe |
5Ž -FA M)- AA AgA AgA TgC gCC TAC AAg ACC TgT TgC -( TAM RA) -3Å ½ |
29 |
72.1 (PCR: 58) |
68.0 |
86 |
48.28 |
|
|
| NCBI-Acc: |
|
Sequence Description: |
Tilia platyphyllos nitrate reductase (nr1) mRNA, complete cds. |
| BaseCount: |
|
| DNA-Sequence: |
|
AminoAcid-Sequence: |
|
|
You are here: Core Forms > Search PCR > PCR
|
Overview