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PCR |
| Name: |
GDF8-SPE |
Type: |
PCR-RFLP |
System: |
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| Genetic Element: |
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Ampl. Length: |
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Ampl. Position: |
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Sequence Size [bp]: |
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| AnnTemp [°C]: |
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Taxonomy: |
Cattle (Beef (general)) |
Temp. Time Program: |
Cycling conditions :<br>One cycle of DNA denaturation for 2 min at 94 °C;<br>35 three-step cycles 94 °C for 1 min<br>56 °C for 1 min<br>and 72 °C for 1 min 30 s<br>Last extension for 10 min at 72 ° |
| Device: |
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Reagents: |
Primer 1 (10 μM) 4 μl<br>Primer 2 (10 μM) 4 μl<br>PCR buffer 10x 5 μl<br>MgCl2 (25 mM) 3 μl<br>dNTPs (2 mM) 2,5 μl<br>Taq DNA polymerase (5 U/μl) 0,4 μl<br>H2O<br>ADN 100 ng<br>Total volume 50 μl |
nPrimers: |
2 |
Organisation: |
CRA-W |
| PCR Description: |
PCR-RFLP protocol used for the genotyping of the Myostatin promoter according to Crisà et al. (2003) |
Sequence: |
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| LOD [copy]: |
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LOQ [copy]: |
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Efficiency [%]: |
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| Remarks: |
PCR-RFLP protocol used for the genotyping of the Myostatin promoter according to Crisà et al. (2003) |
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
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GDF8-SPE-F |
Sense |
gCT CCC AgA CCT TAC CCC AAA TC |
23 |
71.2 |
62.4 |
72 |
56.52 |
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GDF8-SPE-R |
Antisense |
gTT gAT ATg CTT TTC CCT CAg |
21 |
61.1 |
52.3 |
60 |
42.86 |
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