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PCR |
| Name: |
A-allele |
Type: |
PCR |
System: |
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| Genetic Element: |
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Ampl. Length: |
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Ampl. Position: |
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Sequence Size [bp]: |
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| AnnTemp [°C]: |
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Taxonomy: |
Cattle (Beef (general)) |
Temp. Time Program: |
Cycling conditions :<br>One cycle of DNA denaturation for 2 min at 94 °C;<br>35 three-step cycles 94 °C for 30 sec<br>61 °C for 30 sec<br>and 72 °C for 1 min<br>Last extension for 3 min at 72 °C |
| Device: |
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Reagents: |
PCR mix:<br>Primer 1 (10 μM) 1 μl<br>Primer 2 (10 μM) 1 μl<br>PCR buffer 10x 2,5 μl<br>MgCl2 (25 mM) 3 μl<br>dNTPs (2 mM) 2,5 μl<br>Taq DNA polymerase (5 U/μl) 0,4 μl<br>H2O<br>ADN 100 ng<br>Total volume 25 μl |
nPrimers: |
2 |
Organisation: |
CRA-W |
| PCR Description: |
PCR protocol used for the detection of the A, Abr alleles of the Agouti gene |
Sequence: |
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| LOD [copy]: |
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LOQ [copy]: |
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Efficiency [%]: |
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| Remarks: |
PCR protocol used for the detection of the A, Abr alleles of the Agouti gene |
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
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AREP16 |
Sense |
Agg CAA CAC TAA gTC ACA ACC CTg gTC TT |
30 |
72.1 |
68.1 |
90 |
50.00 |
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AREP25 |
Antisense |
gAT Tgg AgC AAg Agg gTT AAg TgA AAC TCT |
30 |
72.1 |
64.0 |
86 |
43.33 |
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