|
You are here: Core Forms > Search PCR > PCR
 First |  Previous | | Next  | Last  |
PCR |
| Name: |
Parth2 |
Type: |
PCR |
System: |
|
| Genetic Element: |
|
Ampl. Length: |
|
Ampl. Position: |
|
Sequence Size [bp]: |
|
| AnnTemp [°C]: |
|
Taxonomy: |
Cattle (Beef (general)) |
Temp. Time Program: |
Cycling conditions :<br>One cycle of DNA denaturation for 4 min at 95 °C;<br>50 three-step cycles 94 °C for 15 sec<br>60 °C for 15 sec<br>and 72 °C for 15 sec<br>Last extension for 6 min at 72 °C |
| Device: |
|
Reagents: |
PCR mix:<br>Primer M3-F (5 μM) 2 μl<br>Primer Parth2-R (5 μM) 2 μl<br>PCR buffer 10x 2 μl<br>MgCl2 (25 mM) 2 μl<br>dNTPs (2 mM) 2 μl<br>Taq DNA polymerase (5 U/μl) 0,1 μl<br>H2O<br>ADN 100 ng<br>Total volume 20 μl |
nPrimers: |
2 |
Organisation: |
CRA-W |
| PCR Description: |
Original PCR protocol developed by CRA-W and INRA for the detection of the E1 allele of the MC1R gene |
Sequence: |
|
| LOD [copy]: |
|
LOQ [copy]: |
|
Efficiency [%]: |
|
| Remarks: |
Original PCR protocol developed by CRA-W and INRA for the detection of the E1 allele of the MC1R gene |
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
M3-F |
Sense |
CAT AgC TAT gCT ggC CCT gAT |
21 |
66.2 |
57.4 |
64 |
52.38 |
|
|
Parth2-R |
Antisense |
gCg CTg CCT CTT CTg gAg |
18 |
67.7 |
58.9 |
60 |
66.67 |
|
|
You are here: Core Forms > Search PCR > PCR
|
Overview