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Primer |
| PPName: |
act-probe |
Type: |
Marker/Probe |
PrimerSequence: |
FAM - CCT CCA ATC CAg ACA CTg TAC TTT CTC TC - T AMR A |
| Length [bp]: |
29 |
T_Ann [°C]: |
70.9 |
TmThermo [°C]: |
62.2 |
Tm2AT [°C]: |
86 |
GC [%]: |
48.28 |
| Position: |
|
Sequence: |
AY372368 |
Dye: |
6FAM (5-FAM) |
PCR: |
act |
| Remarks: |
|
|
| Name: |
|
Type: |
Real-Time PCR |
System: |
Quantitative |
Sequence: |
AY372368 |
| Genetic Element: |
Actin |
Ampl. Length: |
103 |
Ampl. Position: |
|
Sequence Size [bp]: |
|
| LOD [copy]: |
1 |
LOQ [copy]: |
10 |
Efficiency [%]: |
107 |
|
| NCBI-Acc: |
|
Sequence Description: |
Trifolium pratense actin mRNA, complete cds. |
| BaseCount: |
|
| DNA-Sequence: |
|
AminoAcid-Sequence: |
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