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Primer |
| PPName: |
Til_nr-probe |
Type: |
Marker/Probe |
PrimerSequence: |
5Ž -FA M)- AA AgA AgA TgC gCC TAC AAg ACC TgT TgC -( TAM RA) -3Å ½ |
| Length [bp]: |
29 |
T_Ann [°C]: |
72.1 (PCR: 58) |
TmThermo [°C]: |
68.0 |
Tm2AT [°C]: |
86 |
GC [%]: |
48.28 |
| Position: |
|
Sequence: |
AY138811 |
Dye: |
6FAM (5-FAM) |
PCR: |
Til_nr |
| Remarks: |
|
|
| Name: |
|
Type: |
Real-Time PCR |
System: |
Quantitative |
Sequence: |
AY138811 |
| Genetic Element: |
nitrate reductase |
Ampl. Length: |
101 |
Ampl. Position: |
|
Sequence Size [bp]: |
|
| LOD [copy]: |
25 |
LOQ [copy]: |
250 |
Efficiency [%]: |
98 |
|
| NCBI-Acc: |
|
Sequence Description: |
Tilia platyphyllos nitrate reductase (nr1) mRNA, complete cds. |
| BaseCount: |
|
| DNA-Sequence: |
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AminoAcid-Sequence: |
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