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Primer |
| PPName: |
Cas_PAL-probe |
Type: |
Marker/Probe |
PrimerSequence: |
(FA M)A AAG CAG CAA TTC CTA ACC CAA TTA AGG AGT (TA MRA ) |
| Length [bp]: |
31 |
T_Ann [°C]: |
72.1 (PCR: 60) |
TmThermo [°C]: |
65.4 |
Tm2AT [°C]: |
86 |
GC [%]: |
38 |
| Position: |
|
Sequence: |
|
Dye: |
6FAM (5-FAM) |
PCR: |
Cas_PAL |
| Remarks: |
|
|
| Name: |
|
Type: |
Real-Time PCR |
System: |
Quantitative |
Sequence: |
|
| Genetic Element: |
phenylalanine ammonia-lyase (PAL) |
Ampl. Length: |
144 |
Ampl. Position: |
|
Sequence Size [bp]: |
|
| LOD [copy]: |
10 |
LOQ [copy]: |
100 |
Efficiency [%]: |
90 |
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