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Primer |
PPName: |
Cas_PAL-r |
Type: |
Antisense |
PrimerSequence: |
gAA TTC TCT CAC CAg TTA gTA AAC TTg TT |
Length [bp]: |
29 |
T_Ann [°C]: |
65.5 (PCR: 60) |
TmThermo [°C]: |
56.7 |
Tm2AT [°C]: |
78 |
GC [%]: |
34.48 |
Position: |
|
Sequence: |
|
Dye: |
- |
PCR: |
Cas_PAL |
Remarks: |
|
|
Name: |
|
Type: |
Real-Time PCR |
System: |
Quantitative |
Sequence: |
|
Genetic Element: |
phenylalanine ammonia-lyase (PAL) |
Ampl. Length: |
144 |
Ampl. Position: |
|
Sequence Size [bp]: |
|
LOD [copy]: |
10 |
LOQ [copy]: |
100 |
Efficiency [%]: |
90 |
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