|
You are here: Core Forms > Search PCR > PCR
 First |  Previous | | Next  | Last  |
PCR |
| Name: |
CerQual |
Type: |
PCR |
System: |
Qualitative |
| Genetic Element: |
|
Ampl. Length: |
|
Ampl. Position: |
|
Sequence Size [bp]: |
|
| AnnTemp [°C]: |
|
Taxonomy: |
Cereals: Oat-Barley-Rye-Wheat |
Temp. Time Program: |
Denaturation PCR (45 cycles) Hold<br>95°C : 10 min 95°C : 30 sec 72°C : 5 min<br> 65°C : 30 sec<br> 72°C : 60 sec |
| Device: |
|
Reagents: |
Reagent Volume/tube<br>H2O PCR grade 26.5 μL<br>MgCl2 (25mM) 3 μL<br>Takara (10X) buffer 5 μL<br>BSA (0.02μg/μl) 5 μL<br>dNTP Takara (2.5mM) 5 μL<br>Primer Cer1F (20μM) 1 μL<br>Primert Cer2R (20μM) 1 μL<br>Takara Taq Polymerase<br>(5 unités/μl) 0.5 μL<br>DNA 3 μL<br>Volume total 50 μL |
nPrimers: |
4 |
Organisation: |
Eurofins |
| PCR Description: |
|
Sequence: |
|
| LOD [copy]: |
|
LOQ [copy]: |
|
Efficiency [%]: |
|
| Remarks: |
|
Food/Product |
| Food Name: |
Generic Image: |
Product Type: |
Food Source: |
Product Website: |
| Wheat |  | Cereals and cereal products [A0457] | | |
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
Cer1F |
Sense |
AAT CCT gAg CCA AAT CCg Tg |
20 |
66.9 |
58.1 |
60 |
50.00 |
|
|
Cer2R |
Antisense |
CAT gTA gAA Tgg gAC TCT CTC TTT g |
25 |
64.8 |
56.0 |
72 |
44.00 |
|
|
FluoCer1 |
Marker/Probe |
5&q uot ;-T AAC TCg ATT CgT TAg AAC AgC TTC CAT TgA gTC TCT -3 &qu ot; FL |
37 |
|
|
|
|
|
|
RedCer1 |
Marker/Probe |
5&q uot ;-L C R ed6 40 - C CTA TCC TTT TCC TTT gTA TTC TAg TTC gAg A - PH |
32 |
|
|
|
|
|
|
You are here: Core Forms > Search PCR > PCR
|
Overview