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PCR

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PCR
Name:

plant-nes-2

 Type: Real-Time PCR System: Quantitative
Genetic Element: Ampl. Length: 103 Ampl. Position: Sequence Size [bp]:
AnnTemp [°C]: Taxonomy: Plants Temp. Time Program:
Device: Reagents: nPrimers: 3 Organisation: BfR
PCR Description: Sequence:
LOD [copy]: 0.1 LOQ [copy]: 1 Efficiency [%]: 98
Remarks:

Food/Product
Food Name: Generic Image: Product Type: Food Source: Product Website:
Miel de CorseSUGAR AND HONEY [A0462]INSECT [B1220]http://europa.e... /en/1129_en.htm

Primers
PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
plant nes-2-f Sense ATT gAg CCT Tgg TAT ggA AAC CT 23 67.6 58.8 66 43.48
plant nes-2-r Antisense ggA TTT ggC TCA ggA TTg CC 20 68.2 59.5 62 55.00
plant nes-2-probe Marker/Probe 5Ž -FA M - TT AAT TCC AGG GTT TCT CTG AAT TTG AAA GTT -( TAM RA) -3Å ½ 32

Publications (more...)
Short Title Type Date Download
SOP PlantSpecies by PCR SOP September 2007 SOP_BfR_species-detection_071016.pdf
Analytical Methods: Detection of plant species in honey Journal Accepted: 22.09.2008 FoodChemistry_118_979-986_Laube_Honey_2010.pdf

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