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You are here: Core Forms > Search PCR > PCR

PCR

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PCR
Name:

Querc_PAL2

 Type: Real-Time PCR System: Quantitative
Genetic Element: phenylalanine ammonia-lyase (PAL) Ampl. Length: 141 Ampl. Position: Sequence Size [bp]:
AnnTemp [°C]: 60 Taxonomy: Oak: Cork oak (Quercus suber) Temp. Time Program: Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60<br>
Device: Reagents: nPrimers: 3 Organisation: BfR
PCR Description: Sequence: AY443341
LOD [copy]: 10 LOQ [copy]: 100 Efficiency [%]: 91
Remarks:

Food/Product
Food Name: Generic Image: Product Type: Food Source: Product Website:
Miel de CorseSUGAR AND HONEY [A0462]INSECT [B1220]http://europa.e... /en/1129_en.htm

Primers
PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
Querc_PAL2-f Sense AAg AAg TAg Agg gCg CAA gg 20 66.9 (PCR: 60) 58.1 62 55.00
Querc_PAL-r Antisense gAA TTC TCT CAC CAg TTA gTA AAC TTg TT 29 65.5 (PCR: 60) 56.7 78 34.48
Querc_PAL-probe Marker/Probe 5Ž -FA M)- AA AgC AgC AAT TCC TAA CCC AAT TAA ggA gT -(T AMR A)- 3Ž 31 PCR: 60 86

Sequence (more...)
NCBI-Acc:

AY443341

 Sequence Description: Quercus suber phenylalanine ammonia-lyase (PAL) mRNA, complete cds.
BaseCount:
DNA-Sequence: AminoAcid-Sequence:

Publications (more...)
Short Title Type Date Download
SOP PlantSpecies by PCR SOP September 2007 SOP_BfR_species-detection_071016.pdf
Analytical Methods: Detection of plant species in honey Journal Accepted: 22.09.2008 FoodChemistry_118_979-986_Laube_Honey_2010.pdf

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