PCR |
| Name: |
ApisDorsata_RT |
Type: |
Real-Time PCR |
System: |
Qualitative |
| Genetic Element: |
18S rRNA |
Ampl. Length: |
101 |
Ampl. Position: |
352-452 |
Sequence Size [bp]: |
462.Apis dorsana mitochondr.16S rRNA large subunit |
| AnnTemp [°C]: |
55-59 |
Taxonomy: |
Rock Honeybee |
Temp. Time Program: |
UNG activity: <br>2 min. / 50 °C<br>Activation of HotStarTaq: <br>10 min. / 95 °C<br>Amplification (50 cycles): <br>15 sec. / 95 °C <br>60 sec. / 60 °C<br> |
| Device: |
ABI Prism® Sequence Detection System 7700, Applied Biosystems |
Reagents: |
2x RT PCR Master Mix; Forward Primer 320 nM;<br>Reverse Primer 320 nM; Probe 160 nM; H2O |
nPrimers: |
3 |
Organisation: |
Biolytix |
| PCR Description: |
Real-Time PCR for the detection of Apis Dorsata. Apis dorsata mitochondrial ribosomal RNA large subunit (16S rRNA) gene fragment (462). |
Sequence: |
Apis.Mellifera_18S_rRNA |
| LOD [copy]: |
|
LOQ [copy]: |
|
Efficiency [%]: |
|
| Remarks: |
With the method describe it is possible to detect DNA from Apis cerana, Apis mellifera, Apis florae and Apis dorsata with real-time PCR . The real-time PCR systems are based on detection of mitochondrial rRNA. This method was designed by Mandy Schöne-Michling. |
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