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PCR

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PCR
Name:

Bos-pde MolSpec

 Type: Real-Time PCR System: Quantitative
Genetic Element: Phosphodiesterase Ampl. Length: 154 Ampl. Position: Sequence Size [bp]:
AnnTemp [°C]: 60 Taxonomy: Cattle (Beef (general)) Temp. Time Program:
Device: Reagents: nPrimers: 4 Organisation: BfR
PCR Description: Sequence: M27541
LOD [copy]: LOQ [copy]: Efficiency [%]:
Remarks: Test Record from the MolSpec-Project.
Not part of the TRACE-project
Reference Sequence: M27541

Food/Product
Food Name: Generic Image: Product Type: Food Source: Product Website:
MEAT AND MEAT PRODUCTS [A0459]COW [B1201]http://europa.e... l/en/501_en.htm

Primers
PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
Bos-pde-f Sense ACA CAA TgA ATT gAC TCC TAC CC 23 64.1 55.4 66 43.5 2994-3016
Bos-pde-r Antisense ggT gCg TAA Tgg Agg AgT C 19 63.1 54.3 60 57.9 3147-3129
Bos-pde-probe Marker/Probe ATA TgC CTg gAg TTT gTT TTT AAA TAT TTC AgC 33 71.9 63.1 86 30.3 3124-3092
Bos-pde-Amplicon Amplicon aca caa tga att gAC TCC TAC CCA TCA TGC AGA Ttc taa cat cag gat ttt tgc tgc att tgc ttc atc tgt cct ttt aga gtt ttc ttT TTT TCT TAG CTG AAA TAT TTA AAA ACA aac tcc agg cat atc att GAC TCC TCC ATT ACG CAC C 154 60 87.5 420 36.4 2994-3147

Sequence (more...)
NCBI-Acc:

M27541

 Sequence Description: Bovine cyclic GMP phosphodiesterase alpha subunit mRNA, complete cds.
BaseCount: 887 a 783 c 798 g 733 t
DNA-Sequence: M27541.txt AminoAcid-Sequence: M27541transl.txt

Publications (more...)
Short Title Type Date Download
Simultaneous PCR-detection of animal species in foods Journal January 2007 http://www3.interscience.wiley.com/cgi-bin/fulltext/117989001/PDFSTART
Real-time PCR-detection of beef and pork in foods Journal February 2003 http://www3.interscience.wiley.com/cgi-bin/fulltext/118888338/PDFSTART
Quantitative Real-time PCR Detection of Animal species in foods Journal March 2007 http://www3.interscience.wiley.com/cgi-bin/fulltext/117989096/PDFSTART

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