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PCR |
| Name: |
M1 |
Type: |
PCR-RFLP |
System: |
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| Genetic Element: |
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Ampl. Length: |
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Ampl. Position: |
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Sequence Size [bp]: |
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| AnnTemp [°C]: |
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Taxonomy: |
Cattle (Beef (general)) |
Temp. Time Program: |
Cycling conditions :<br>One cycle of DNA denaturation for 5 min at 94 °C;<br>35 three-step cycles 94 °C for 30 sec<br>61 °C for 30 sec<br>and 72 °C for 1 min<br>Last extension for 5 min at 72 °C |
| Device: |
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Reagents: |
Primer M1 (5 μM) 1,6 μl<br>Primer M2 (5 μM) 1,6 μl<br>PCR buffer 10x 5 μl<br>MgCl2 (25 mM) 3 μl<br>dNTPs (2 mM) 5 μl<br>Taq DNA polymerase (5 U/μl) 0,4 μl<br>H2O<br>ADN 100 ng<br>Total volume 50 μ |
nPrimers: |
2 |
Organisation: |
CRA-W |
| PCR Description: |
PCR-RFLP protocol used for the detection of the E, Ed and e alleles of the MC1R gene<br>according to Rolando and Di Stasio (2006) |
Sequence: |
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| LOD [copy]: |
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LOQ [copy]: |
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Efficiency [%]: |
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| Remarks: |
PCR-RFLP protocol used for the detection of the E, Ed and e alleles of the MC1R gene
according to Rolando and Di Stasio (2006) |
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
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M1 |
Sense |
AAg AAC CgC AAC CTg CAC T |
19 |
67.3 |
58.6 |
58 |
52.63 |
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M2 |
Antisense |
CTC gTT ggC CTC TTC ATA gC |
20 |
64.9 |
56.2 |
62 |
55.00 |
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