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PCR |
| Name: |
WBR MolSpec |
Type: |
PCR-RFLP |
System: |
Qualitative (Light Cycler; TaqMan; Matsunaga) |
| Genetic Element: |
Intron of trnL gene |
Ampl. Length: |
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Ampl. Position: |
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Sequence Size [bp]: |
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| AnnTemp [°C]: |
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Taxonomy: |
Wheat |
Temp. Time Program: |
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| Device: |
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Reagents: |
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nPrimers: |
2 |
Organisation: |
BATS |
| PCR Description: |
Taxon: Detection of Wheat, Barley, Rye. Publication: Dahinden I., von Büren M., Lüthy J. (2001) A quantitative competitive PCR system to detect contamination of wheat, barley or rye in gluten-free food for coeliac patients. Eur. Food Res. Technol. 212, 228-233. |
Sequence: |
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| LOD [copy]: |
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LOQ [copy]: |
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Efficiency [%]: |
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| Remarks: |
Test Record from the MolSpec-Project.
Not part of the TRACE-project
Taxon: Only Wheat, Barley, Rye. Publication: Dahinden I., von Büren M., Lüthy J. (2001) A quantitative competitive PCR system to detect contamination of wheat, barley or rye in gluten-free food for coeliac patients. Eur. Food Res. Technol. 212, 228-233. |
Food/Product |
| Food Name: |
Generic Image: |
Product Type: |
Food Source: |
Product Website: |
| Wheat |  | Cereals and cereal products [A0457] | | |
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
WBR11 |
Sense |
ggT AAC TTC CAA ATT CAg AgA AAC |
24 |
62.7 |
53.9 |
66 |
37.5 |
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WBR13 |
Antisense |
TCT CTA ATT TAg AAT TAg AAg gAA |
24 |
55.7 |
46.9 |
60 |
25.0 |
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