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PCR

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PCR
Name:

TGLA227(D18S1)

 Type: Microsatellite System:
Genetic Element: Ampl. Length: Ampl. Position: C18: 75-105 Sequence Size [bp]:
AnnTemp [°C]: 55-56 Taxonomy: Cattle (Beef (general)) Temp. Time Program:
Device: Reagents: nPrimers: 2 Organisation: UU-FDG
PCR Description: Sequence:
LOD [copy]: LOQ [copy]: Efficiency [%]:
Remarks: Markers are also used in the Stockmarket kit from ABI for routine parentage testing

Food/Product
Food Name: Generic Image: Product Type: Food Source: Product Website:
Beef meatMEAT AND MEAT PRODUCTS [A0459]COW [B1201]http://europa.e... l/en/501_en.htm

Primers
PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
TGLA227-f Sense CGA ATT CCA AAT CTG TTA ATT TGC T 25 55-56
TGLA227-r Antisense ACA GAC AGA AAC TCA ATG AAA GCA 24 55-56

Publications (more...)
Short Title Type Date Download
SOP for microsatellite based bovine breed SOP September 2007 SOP_UU_070903-SOPmst.pdf
European Genetic Cattle Diversity Consortium Journal October 2006 http://www3.interscience.wiley.com/cgi-bin/fulltext/118596266/PDFSTART
A Software for Genetic Assignment Journal 2004
Breed assignment of cattle by AFLP markers Journal April 2007 http://www3.interscience.wiley.com/cgi-bin/fulltext/118504276/PDFSTART
Cattle differentiation by AFLP fingerprinting Journal February 2007 http://www3.interscience.wiley.com/cgi-bin/fulltext/118504258/PDFSTART

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