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PCR |
| Name: |
Bras_lip |
Type: |
Real-Time PCR |
System: |
Quantitative |
| Genetic Element: |
Lipase 1 |
Ampl. Length: |
122 |
Ampl. Position: |
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Sequence Size [bp]: |
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| AnnTemp [°C]: |
57 |
Taxonomy: |
Rape, Canola, Rapeseed, Oilseed Rape, Colza |
Temp. Time Program: |
Process Time [min:s] Temperature [°C]<br>Pre-PCR: decontamination 02:00 50<br>Pre-PCR: activation of DNA polymerase and denaturation of template DNA 10:00 95<br>PCR (45 cycles) <br>Step 1 Denaturation 00:15 95<br>Step 2 Annealing and elongation 01:00 60 |
| Device: |
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Reagents: |
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nPrimers: |
3 |
Organisation: |
BfR |
| PCR Description: |
|
Sequence: |
AY866419 |
| LOD [copy]: |
1 |
LOQ [copy]: |
10 |
Efficiency [%]: |
102 |
| Remarks: |
|
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
Bras_lip-f2 |
Sense |
CAC gAC CAC gTT CTT TgT TTT |
21 |
66.0 (PCR: 57) |
57.3 |
60 |
42.86 |
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Bras_lip-r |
Antisense |
CCA CTA CAA CAT TCC ATC CCC |
21 |
66.0 (PCR: 57) |
57.2 |
64 |
52.38 |
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Bras_lip-probe |
Marker/Probe |
5Ž -FA M)- TC ACC AAA ACC gCA gCA AgC A - (TA MRA )-3 Ž |
21 |
72.1 (PCR: 57) |
65.4 |
64 |
52.38 |
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| NCBI-Acc: |
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Sequence Description: |
Brassica napus lipase 1 mRNA, complete cds. |
| BaseCount: |
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| DNA-Sequence: |
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AminoAcid-Sequence: |
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