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Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
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Apis |
cerana |
Insecta |
Hymenoptera |
Apidae |
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Name: |
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Type: |
Real-Time PCR |
System: |
Qualitative |
Sequence: |
Apis.Mellifera_18S_rRNA |
Genetic Element: |
18S rRNA |
Ampl. Length: |
113 |
Ampl. Position: |
358-470 |
Sequence Size [bp]: |
470.Apis cerana mitochondr.16S rRNA large subunit |
LOD [copy]: |
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LOQ [copy]: |
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Efficiency [%]: |
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PCR Description: |
Real-Time PCR for the detection of Apis cerana |
Temp. Time Program: |
UNG activity: <br>2 min. / 50 °C<br>Activation ... (more) |
Primers |
PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
A.cera-F |
Sense |
CCT TAg gAT AAC AgC gTA ATA T |
22 |
57.8 |
49.1 |
60.0 |
36.4 |
358-379 |
A.cera-R |
Antisense |
CAg ACT TAA AAT TTT AAA CTC CT |
23 |
57.4 |
48.6 |
58.0 |
26.1 |
448-470 |
A.cera-probe |
Marker/Probe |
TTg ATA gAC CAC ATT gAT AAA gAT gT |
26 |
61.9 |
53.1 |
68.0 |
30.8 |
383-408 |
Organisation
(more...) |
Acronym: |
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Website: |
http://www.biolytix.ch/ |
Name: |
Biolytix AG |
Country: |
Switzerland |
Postal Address: |
Biolytix AG<br>Peter Brodmann<br>Benkenstrasse 254<br>4108 Witterswil |
Phone: |
+41 (0)61 725 20 70 |
Fax: |
+41 (0)61 725 20 71 |
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