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Overview

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Food/Product
Food Name: Generic Image: Product Type: Food Source: Product Website:
Miel de CorseSUGAR AND HONEY [A0462]INSECT [B1220]http://europa.e... /en/1129_en.htm

Taxonomy (more...)

Popular Name: Genus: Species: Class: Order: Family:

Olive

 Olea europaea Magnoliopsida Lamiales Oleaceae

Sequence (more...)
NCBI-Acc:

AY082335

 Sequence Description: Olea europaea allergen Ole e 10 mRNA, complete cds.
BaseCount:
DNA-Sequence: AminoAcid-Sequence:

PCR (more...)
Name:

Olea_ole10

 Type: Real-Time PCR System: Quantitative Sequence: AY082335
Genetic Element: Allergen Ole e 10 Ampl. Length: 96 Ampl. Position: Sequence Size [bp]:
LOD [copy]: 25 LOQ [copy]: 250 Efficiency [%]: 99
PCR Description: Temp. Time Program: Process Time [min:s] Temperature [°C]<br>Pre-PCR: dec... (more)

Primers
PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
Olea_ole10-f Sense TAC CAA AGC AAG GGT CGA AAT G 22 68.2 (PCR: 60) 59.5 64 45.45
Olea_ole10-r2 Antisense AGG GAT AAA ATT GTT TTA CTT ACT TGG GT 29 68.0 (PCR: 60) 59.3 76 31.03
Olea_ole10-probe Marker/Probe (FA M)T CGA TTG CGA CTT TTC GGG CAC C(T AMR A) 23 72.1 (PCR: 60) 68.6 72 56.52

Publications (more...)
Short Title Type Date Download
SOP PlantSpecies by PCR SOP September 2007 SOP_BfR_species-detection_071016.pdf
Analytical Methods: Detection of plant species in honey Journal Accepted: 22.09.2008 FoodChemistry_118_979-986_Laube_Honey_2010.pdf

Organisation (more...)
Acronym:

BfR

 BfR Website: http://www.bfr.bund.de/
Name: BfR-Federal Institute for Risk Assessment, Berlin Country: Germany
Postal Address: Thielallee 88-92, D-14195 Berlin Phone: +49-30-8412-0 Fax: +41 308412 3685

Devices
Electrophor. RT-device DNA-Seqencer ThermoCyl. SouthernBlot Other
DNA+protein ABI 7700+LC ABI 310 PE 2400 yes ELISA plate reader

Samples
Trace Sample Code Producer Food Product Name Sampling Date Sampling Site
3HXXXXX00000145 C. Giudicelli Miel de Corse 2031-05-20
3HXXXXX00000146 Ms Mattei Miel de Corse 2031-05-20
3HXXXXX00000149 J.Cordoliani Miel de Corse 2031-05-20

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