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Overview

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Food/Product
Food Name: Generic Image: Product Type: Food Source: Product Website:
MEAT AND MEAT PRODUCTS [A0459]COW [B1201]http://europa.e... l/en/501_en.htm

Taxonomy (more...)

Popular Name: Genus: Species: Class: Order: Family:

Cattle (Beef (general))

 Bos taurus Eutheria Ruminantia Bovidae

Sequence (more...)
NCBI-Acc:

M27541

 Sequence Description: Bovine cyclic GMP phosphodiesterase alpha subunit mRNA, complete cds.
BaseCount: 887 a 783 c 798 g 733 t
DNA-Sequence: M27541.txt AminoAcid-Sequence: M27541transl.txt

PCR (more...)
Name:

Bos-pde0 MolSpec

 Type: Real-Time PCR System: Quantitative Sequence: M27541
Genetic Element: Phosphodiesterase Ampl. Length: 104 Ampl. Position: Sequence Size [bp]:
LOD [copy]: LOQ [copy]: Efficiency [%]:
PCR Description: Temp. Time Program: more)

Primers
PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
Bos-f Sense ACT CCT ACC CAT CAT gCA gAT 21 63.1 54.3 62 47.6 3007-3027
Bos-r Antisense TgT TTT TAA ATA TTT CAg CTA AgA AAA A 28 63.1 54.3 66 17.9 3110-3083
Bos-probe Marker/Probe AAC ATC Agg ATT TTT gCT gCA TTT gC 26 72.1 63.8 72 38.5 3031-3056
Bos-Amplicon Amplicon ACT CCT ACC CAT CAT GCA GAT tct aac atc agg att ttt gct gca ttt gct tca tct gtc ctt tta gag ttt tct tTT TTT CTT AGC TGA AAT ATT TAA AAA CA 104 60 82.8 276 32.7 3007-3110

Publications (more...)
Short Title Type Date Download
Simultaneous PCR-detection of animal species in foods Journal January 2007 http://www3.interscience.wiley.com/cgi-bin/fulltext/117989001/PDFSTART
Real-time PCR-detection of beef and pork in foods Journal February 2003 http://www3.interscience.wiley.com/cgi-bin/fulltext/118888338/PDFSTART
Quantitative Real-time PCR Detection of Animal species in foods Journal March 2007 http://www3.interscience.wiley.com/cgi-bin/fulltext/117989096/PDFSTART

Organisation (more...)
Acronym:

BfR

 BfR Website: http://www.bfr.bund.de/
Name: BfR-Federal Institute for Risk Assessment, Berlin Country: Germany
Postal Address: Thielallee 88-92, D-14195 Berlin Phone: +49-30-8412-0 Fax: +41 308412 3685

Devices
Electrophor. RT-device DNA-Seqencer ThermoCyl. SouthernBlot Other
DNA+protein ABI 7700+LC ABI 310 PE 2400 yes ELISA plate reader

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