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Overview

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Food/Product
Food Name: Generic Image: Product Type: Food Source: Product Website:
Miel de CorseSUGAR AND HONEY [A0462]INSECT [B1220]http://europa.e... /en/1129_en.htm

Taxonomy (more...)

Popular Name: Genus: Species: Class: Order: Family:

Oak: Cork oak (Quercus suber)

 Quercus suber Rosids Fagales Fagaceae

Sequence (more...)
NCBI-Acc:

AY443341

 Sequence Description: Quercus suber phenylalanine ammonia-lyase (PAL) mRNA, complete cds.
BaseCount:
DNA-Sequence: AminoAcid-Sequence:

PCR (more...)
Name:

Querc_PAL2

 Type: Real-Time PCR System: Quantitative Sequence: AY443341
Genetic Element: phenylalanine ammonia-lyase (PAL) Ampl. Length: 141 Ampl. Position: Sequence Size [bp]:
LOD [copy]: 10 LOQ [copy]: 100 Efficiency [%]: 91
PCR Description: Temp. Time Program: Process Time [min:s] Temperature [°C]<br>Pre-PCR: dec... (more)

Primers
PPName: Type: PrimerSequence: Length [bp]: T_Ann [°C]: TmThermo [°C]: Tm2AT [°C]: GC [%]: Position:
Querc_PAL2-f Sense AAg AAg TAg Agg gCg CAA gg 20 66.9 (PCR: 60) 58.1 62 55.00
Querc_PAL-r Antisense gAA TTC TCT CAC CAg TTA gTA AAC TTg TT 29 65.5 (PCR: 60) 56.7 78 34.48
Querc_PAL-probe Marker/Probe 5Ž -FA M)- AA AgC AgC AAT TCC TAA CCC AAT TAA ggA gT -(T AMR A)- 3Ž 31 PCR: 60 86

Publications (more...)
Short Title Type Date Download
SOP PlantSpecies by PCR SOP September 2007 SOP_BfR_species-detection_071016.pdf
Analytical Methods: Detection of plant species in honey Journal Accepted: 22.09.2008 FoodChemistry_118_979-986_Laube_Honey_2010.pdf

Organisation (more...)
Acronym:

BfR

 BfR Website: http://www.bfr.bund.de/
Name: BfR-Federal Institute for Risk Assessment, Berlin Country: Germany
Postal Address: Thielallee 88-92, D-14195 Berlin Phone: +49-30-8412-0 Fax: +41 308412 3685

Devices
Electrophor. RT-device DNA-Seqencer ThermoCyl. SouthernBlot Other
DNA+protein ABI 7700+LC ABI 310 PE 2400 yes ELISA plate reader

Samples
Trace Sample Code Producer Food Product Name Sampling Date Sampling Site
3HXXXXX00000145 C. Giudicelli Miel de Corse 2031-05-20
3HXXXXX00000146 Ms Mattei Miel de Corse 2031-05-20
3HXXXXX00000149 J.Cordoliani Miel de Corse 2031-05-20

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