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| Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
|
Castanea |
vesca/sativa |
Rosids |
Sapindales |
Sapindaceae |
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| Name: |
|
Type: |
Real-Time PCR |
System: |
Quantitative |
Sequence: |
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| Genetic Element: |
phenylalanine ammonia-lyase (PAL) |
Ampl. Length: |
144 |
Ampl. Position: |
|
Sequence Size [bp]: |
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| LOD [copy]: |
10 |
LOQ [copy]: |
100 |
Efficiency [%]: |
90 |
| PCR Description: |
|
Temp. Time Program: |
Process Time [min:s] Temperature [°C]<br>Pre-PCR: dec... (more) |
Primers |
| PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
|
Cas_PAL-f |
Sense |
CCA AAg AAg TAg Agg gTg CAA gA |
23 |
67.2 (PCR: 60) |
58.4 |
68 |
47.83 |
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|
Cas_PAL-r |
Antisense |
gAA TTC TCT CAC CAg TTA gTA AAC TTg TT |
29 |
65.5 (PCR: 60) |
56.7 |
78 |
34.48 |
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|
Cas_PAL-probe |
Marker/Probe |
(FA M)A AAG CAG CAA TTC CTA ACC CAA TTA AGG AGT (TA MRA ) |
31 |
72.1 (PCR: 60) |
65.4 |
86 |
38 |
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Organisation
(more...) |
| Acronym: |
|
 |
Website: |
http://www.bfr.bund.de/ |
| Name: |
BfR-Federal Institute for Risk Assessment, Berlin |
Country: |
Germany |
| Postal Address: |
Thielallee 88-92, D-14195 Berlin |
Phone: |
+49-30-8412-0 |
Fax: |
+41 308412 3685 |
Devices |
| Electrophor. |
RT-device |
DNA-Seqencer |
ThermoCyl. |
SouthernBlot |
Other |
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DNA+protein |
ABI 7700+LC |
ABI 310 |
PE 2400 |
yes |
ELISA plate reader |
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You are here: Core Forms > Search Overview > Overview
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Overview