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Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
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Apis |
mellifera |
Insecta |
Hymenoptera |
Apidae |
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Name: |
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Type: |
Real-Time PCR |
System: |
Qualitative |
Sequence: |
Apis.Mellifera_18S_rRNA |
Genetic Element: |
18S rRNA |
Ampl. Length: |
103 |
Ampl. Position: |
356-458 |
Sequence Size [bp]: |
467.Apis mellifera mitochondr.16S rRNA large subun |
LOD [copy]: |
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LOQ [copy]: |
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Efficiency [%]: |
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PCR Description: |
Real-Time PCR for the detection of Apis mellifera |
Temp. Time Program: |
UNG activity: <br>2 min. / 50 °C<br>Activation ... (more) |
Primers |
PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
A.melli_RT-F |
Sense |
CTT Agg ATA ACA gCg TAA TAT C |
22 |
55.6 |
46.9 |
60.0 |
36.4 |
356-377 |
A.melli_RT-R |
Antisense |
AAA CTT AAA CTA CTg CgC CTA |
21 |
60.4 |
51.7 |
58.0 |
38.1 |
438-458 |
A.melli_RT-probe |
Marker/Probe |
TAg ACC ATA TAg ATA AAg ATg TTT gC |
26 |
59.6 |
50.8 |
68.0 |
30.8 |
384-409 |
Organisation
(more...) |
Acronym: |
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Website: |
http://www.biolytix.ch/ |
Name: |
Biolytix AG |
Country: |
Switzerland |
Postal Address: |
Biolytix AG<br>Peter Brodmann<br>Benkenstrasse 254<br>4108 Witterswil |
Phone: |
+41 (0)61 725 20 70 |
Fax: |
+41 (0)61 725 20 71 |
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