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Popular Name: |
Genus: |
Species: |
Class: |
Order: |
Family: |
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Acacia |
sp. |
Rosids |
Fabales |
Fabaceae |
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Name: |
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Type: |
Real-Time PCR |
System: |
Quantitative |
Sequence: |
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Genetic Element: |
alcohol dehydrogenase 1 |
Ampl. Length: |
107 |
Ampl. Position: |
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Sequence Size [bp]: |
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LOD [copy]: |
10 |
LOQ [copy]: |
10 |
Efficiency [%]: |
98 |
PCR Description: |
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Temp. Time Program: |
Process Time [min:s] Temperature [°C]<br>Pre-PCR: dec... (more) |
Primers |
PPName: |
Type: |
PrimerSequence: |
Length [bp]: |
T_Ann [°C]: |
TmThermo [°C]: |
Tm2AT [°C]: |
GC [%]: |
Position: |
Aca_adh1-f |
Sense |
Tgg ggA ATg TCC ACA TTg TAA gT |
23 |
67.2 (PCR: 60) |
58.4 |
66 |
43.48 |
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Aca_adh1-r |
Antisense |
Tgg AgA ACC Tgg TCT TgC CA |
20 |
68.7 (PCR: 60) |
59.9 |
62 |
55.00 |
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Aca_adh1-probe |
Marker/Probe |
5Ž -FA M)- AG CAT AAC ACC CCT GTC AGT GTT AAT CCT AAG AA -(T AMR A)- 3Ž |
34 |
PCR: 60 |
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Organisation
(more...) |
Acronym: |
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Website: |
http://www.bfr.bund.de/ |
Name: |
BfR-Federal Institute for Risk Assessment, Berlin |
Country: |
Germany |
Postal Address: |
Thielallee 88-92, D-14195 Berlin |
Phone: |
+49-30-8412-0 |
Fax: |
+41 308412 3685 |
Devices |
Electrophor. |
RT-device |
DNA-Seqencer |
ThermoCyl. |
SouthernBlot |
Other |
DNA+protein |
ABI 7700+LC |
ABI 310 |
PE 2400 |
yes |
ELISA plate reader |
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