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You are here: Core Froms > Search Primer > Primer
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Primer |
PPName |
BIO_SIM |
Type |
Sense |
PrimerSequence |
BIO-g ACCTC CCAgC TCCAT CAAAC ATCTC ATCTT gAgAA A |
Length |
37 |
T_ann |
72.1 |
TmThermo |
72.4 |
Tm2AT |
95 |
%GC |
45.9 |
Position |
58-95 |
Sequence |
X56295 |
Dye |
Biotin? |
ProbeType |
|
PCR |
WP2.2 ELISA-PCR CytochromeB |
PCR |
WP2.2 ELISA-PCR CytochromeB |
Remarks |
modified from Matsunaga et al., 1991.
Cytochrome b (Amplicon length: 117bp).
WP 2.2: Development of ELISA-PCR based detection and quantification systems.
ELISA-PCR system for cattle, pig sheep and horse.
MeltingTempThermo: 72.4 C (E. Carpanetto), 71.8 (Tib-Molbiol Oligo-Shop) |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
cons_back_G_ELISA |
Antisense |
gTCTg ATgTg TAgTg TATTg CTAgg AATAg g |
31 |
68.1 |
59.4 |
88 |
41.9 |
144-174 |
cons_back_M_ELISA |
Antisense |
gTCgg ATgTg TAgTg TATTg CTAgg AATAg g |
31 |
71.3 |
62.5 |
90 |
45.2 |
144-174 |
Bos_misC |
Probe_Antisense |
FL-CC CAggA gggAA CCgCA A |
18 |
71.7 |
62.9 |
60 |
66.7 |
14609-14626 |
Sus_misC_C |
Probe_Antisense |
FL-TA ggATT TgCAA gATCA ggCCg ATg |
25 |
72.1 |
64.3 |
74 |
48 |
57-81 |
Ovi_A_ELISA |
Probe_Antisense |
FL-TA gAATC TgTAA AATTA AgCAA ATgCC TAg |
30 |
66.8 |
58.1 |
78 |
30 |
109-138 |
Equ_misA_G_A |
Probe_Antisense |
FL-gT TAAgA TTTgg AggAT AAggg AgAAT |
27 |
66.3 |
58.5 |
74 |
37 |
57-83 |
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