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You are here: Core Froms > Search Primer > Primer
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Primer |
PPName |
bos_probe_new |
Type |
Probe_Antisense |
PrimerSequence |
CCCAg gAggg AACCg AAATT X |
Length |
20 |
T_ann |
69.6 |
TmThermo |
60.8 |
Tm2AT |
62 |
%GC |
55 |
Position |
14607-14627 |
Sequence |
J01394 |
Dye |
LightCycler red 640 |
ProbeType |
|
PCR |
WP2.1QuaL-Multiplex RealTime-PCRCytochromeB |
PCR |
WP2.1QuaL-Multiplex RealTime-PCRCytochromeB |
Remarks |
X= labelling with LightCycler red 640.
Cytochrome b.
WP 2.1: Development of multiplex-PCR for species in food.
Qualitative multiplex real time PCR for pig, cattle and myostatin on the Light Cycler, Roche. |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
cons_back_G |
Antisense |
gTCTg ATgTg TAgTg TATTg CTAgg AATAg g |
31 |
68.1 |
59.4 |
88 |
41.9 |
144-174 |
cons_back_M |
Antisense |
gTCgg ATgTg TAgTg TATTg CTAgg AATAg g |
31 |
71.3 |
62.5 |
90 |
45.2 |
144-174 |
SIM_F_iFL |
Sense |
gACCT CCCAg CTCCA TCAAA CATCT CATCT TgA X T gAA A |
38 |
72.1 |
72.4 |
95 |
44.7 |
58-95 |
sus_probe |
Probe_Antisense |
CAgAT gCCTA AgAgg gAACC gA X |
22 |
69.3 |
60.6 |
68 |
54.5 |
98-119 |
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