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You are here: Core Froms > Search Primer > Primer
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Primer |
PPName |
Bos-Amplicon |
Type |
Amplicon |
PrimerSequence |
ACTCC TACCC ATCAT GCAGA Ttcta acatc aggat ttttg ctgca tttgc ttcat ctgtc ctttt agagt tttct tTTTT TCTTA GCTGA AATAT TTAAA AACA |
Length |
104 |
T_ann |
60 |
TmThermo |
82.8 |
Tm2AT |
276 |
%GC |
32.7 |
Position |
3007-3110 |
Sequence |
|
Dye |
|
ProbeType |
|
PCR |
Bos-pde0 |
PCR |
Bos-pde0 |
Remarks |
|
PCR |
Name: |
Bos-pde0 |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Sequence: |
M27541 |
Genetic Element: |
Phosphodiesterase |
Ampl.Length: |
104 |
GenomSize: |
|
AnnTemp: |
60 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Bos-f |
Sense |
ACTCC TACCC ATCAT gCAgA T |
21 |
63.1 |
54.3 |
62 |
47.6 |
3007-3027 |
Bos-r |
Antisense |
TgTTT TTAAA TATTT CAgCT AAgAA AAA |
28 |
63.1 |
54.3 |
66 |
17.9 |
3110-3083 |
Bos-probe |
Marker/Probe |
AACAT CAggA TTTTT gCTgC ATTTg C |
26 |
72.1 |
63.8 |
72 |
38.5 |
3031-3056 |
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