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Primer

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Primer
PPName Gal-Oalb-2probe Type Marker/Probe PrimerSequence CAACg CCgTT CTCTT CTTTg gCAgA
Length 25 T_ann 72.1 TmThermo 67.4 Tm2AT 76 %GC 52.0
Position 8220-8244 Sequence Dye ProbeType TaqMan PCR Gal-Oalb-2
PCR Gal-Oalb-2 Remarks
PCR
Name: Gal-Oalb-2 Type: Real-Time PCR System: QuaNtitative Sequence: J00895
Genetic Element: Ovalbumin Ampl.Length: 103 GenomSize: 1.25 AnnTemp: 64
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Gal-Oalb-2f Sense TTCTg TATCA AgCAC ATCgC AA 22 65.4 56.7 62 40.9 8197-8218
Gal-Oalb-2r Antisense gTCTT gTTgg AAggg ACAgA gTT 23 66.5 57.7 68 47.8 8299-8277
Gal-Oalb-2f Amplicon ttctg tatca agcac ATCGC AACCA ACGCC GTtct cttct ttggc agatg tgttt cccct taaaa agaag aaagc tgaaA AACTC TGTCC CTTCC AACAA GAc 103 64 88.8 296 43.7 8197-8299
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