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You are here: Core Froms > Search Primer > Primer
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Primer |
PPName |
Bos-pde-probe |
Type |
Marker/Probe |
PrimerSequence |
ATATg CCTgg AgTTT gTTTT TAAAT ATTTC AgC |
Length |
33 |
T_ann |
71.9 |
TmThermo |
63.1 |
Tm2AT |
86 |
%GC |
30.3 |
Position |
3124-3092 |
Sequence |
|
Dye |
6FAM |
ProbeType |
TaqMan |
PCR |
Bos-pde |
PCR |
Bos-pde |
Remarks |
|
PCR |
Name: |
Bos-pde |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Sequence: |
M27541 |
Genetic Element: |
Phosphodiesterase |
Ampl.Length: |
154 |
GenomSize: |
|
AnnTemp: |
60 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Bos-pde-f |
Sense |
ACACA ATgAA TTgAC TCCTA CCC |
23 |
64.1 |
55.4 |
66 |
43.5 |
2994-3016 |
Bos-pde-r |
Antisense |
ggTgC gTAAT ggAgg AgTC |
19 |
63.1 |
54.3 |
60 |
57.9 |
3147-3129 |
Bos-pde-Amplicon |
Amplicon |
acaca atgaa ttgAC TCCTA CCCAT CATGC AGATt ctaac atcag gattt ttgct gcatt tgctt catct gtcct tttag agttt tcttT TTTTC TTAGC TGAAA TATTT AAAAA CAaac tccag gcata tcatt GACTC CTCCA TTACG CACC |
154 |
60 |
87.5 |
420 |
36.4 |
2994-3147 |
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