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Primer

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Primer
PPName Bos-pde-f Type Sense PrimerSequence ACACA ATgAA TTgAC TCCTA CCC
Length 23 T_ann 64.1 TmThermo 55.4 Tm2AT 66 %GC 43.5
Position 2994-3016 Sequence Dye ProbeType PCR Bos-pde
PCR Bos-pde Remarks
PCR
Name: Bos-pde Type: Real-Time PCR System: QuaNtitative Sequence: M27541
Genetic Element: Phosphodiesterase Ampl.Length: 154 GenomSize: AnnTemp: 60
Primers
PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position
Bos-pde-r Antisense ggTgC gTAAT ggAgg AgTC 19 63.1 54.3 60 57.9 3147-3129
Bos-pde-probe Marker/Probe ATATg CCTgg AgTTT gTTTT TAAAT ATTTC AgC 33 71.9 63.1 86 30.3 3124-3092
Bos-pde-Amplicon Amplicon acaca atgaa ttgAC TCCTA CCCAT CATGC AGATt ctaac atcag gattt ttgct gcatt tgctt catct gtcct tttag agttt tcttT TTTTC TTAGC TGAAA TATTT AAAAA CAaac tccag gcata tcatt GACTC CTCCA TTACG CACC 154 60 87.5 420 36.4 2994-3147
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