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You are here: Core Froms > Search Primer > Primer
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Primer |
PPName |
Ovis-12S-r |
Type |
Antisense |
PrimerSequence |
CTCTC gTgTg gTTgA TATAT gTAAA |
Length |
25 |
T_ann |
61.6 |
TmThermo |
52.8 |
Tm2AT |
68 |
%GC |
36.0 |
Position |
976-952 |
Sequence |
|
Dye |
|
ProbeType |
|
PCR |
Ovis-12S |
PCR |
Ovis-12S |
Remarks |
|
PCR |
Name: |
Ovis-12S |
Type: |
PCR-RFLP |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Sequence: |
NC_001941 |
Genetic Element: |
12S rRNA (?) |
Ampl.Length: |
193 |
GenomSize: |
3.3 |
AnnTemp: |
64 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Ovis-12S-f |
Sense |
gCTAC ATTTT CTACC CAAgA AAAT |
24 |
62.7 |
54.0 |
64 |
33.3 |
784-807 |
Ovis-12S-Amplicon |
Amplicon |
gctac atttt ctacc caaga aaatt taata cgaaa gccat tatga aatta atagc caaag gagga tttag cagta aacta agaat agagt gctta gttga atcag gccat gaagc acgca cacac cgccc gtcac cctcc tcaag taaat atgat atact taaac ctatt tacat atatc aacca cacga gag |
193 |
64 |
87.4 |
532 |
37.8 |
784-976 |
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