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You are here: Core Froms > Search Primer > Primer
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Primer |
PPName |
Capra-12S-Amplicon |
Type |
Amplicon |
PrimerSequence |
TGGGC TACAT TTTCT ACCTT AAGaa aatta atacg aaagc catta tgaaa ttaat gacca aagga ggatt tagta gtaaa ctaag aatag agtgc ttagt tgaat taggc catga agcac gcaca caccg cccgt caccc tcctc aagta aatac aatGC ACTCA AGCCT ATTAA CACG |
Length |
179 |
T_ann |
54 |
TmThermo |
88.3 |
Tm2AT |
498 |
%GC |
39.1 |
Position |
779-957 |
Sequence |
|
Dye |
|
ProbeType |
|
PCR |
Capra-12S |
PCR |
Capra-12S |
Remarks |
|
PCR |
Name: |
Capra-12S |
Type: |
PCR-RFLP |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Sequence: |
NC_005044 |
Genetic Element: |
12S rRNA (?) |
Ampl.Length: |
179 |
GenomSize: |
3.24 |
AnnTemp: |
54 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Capra-12S-f |
Sense |
TgggC TACAT TTTCT ACCTT AAg |
23 |
62.4 |
53.7 |
64 |
39.1 |
779-801 |
Capra-12S-r |
Antisense |
CgTgT TAATA ggCTT gAgTg C |
21 |
63.2 |
54.4 |
62 |
47.6 |
957-937 |
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