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PCR |
Name: |
Ovis-12S |
Type: |
PCR-RFLP |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Genetic Element: |
12S rRNA (?) |
Ampl.Length: |
193 |
GenomSize: |
3.3 |
AnnTemp: |
64 |
Taxonomy: |
Sheep |
PCR Description: |
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Temp. Time Program: |
|
Device: |
|
Master Mix: |
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nPrimers: |
3 |
Organisation: |
BfR |
Validation: |
NoValidationAvailable |
Sequence: |
NC_001941 |
Remarks: |
Reference Sequence: NC_001941 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Ovis-12S-f |
Sense |
gCTAC ATTTT CTACC CAAgA AAAT |
24 |
62.7 |
54.0 |
64 |
33.3 |
784-807 |
Ovis-12S-r |
Antisense |
CTCTC gTgTg gTTgA TATAT gTAAA |
25 |
61.6 |
52.8 |
68 |
36.0 |
976-952 |
Ovis-12S-Amplicon |
Amplicon |
gctac atttt ctacc caaga aaatt taata cgaaa gccat tatga aatta atagc caaag gagga tttag cagta aacta agaat agagt gctta gttga atcag gccat gaagc acgca cacac cgccc gtcac cctcc tcaag taaat atgat atact taaac ctatt tacat atatc aacca cacga gag |
193 |
64 |
87.4 |
532 |
37.8 |
784-976 |
Sequence |
NCBI-Acc: |
NC_001941 |
Sequence Description: |
Ovis aries mitochondrion, complete genome. |
BaseCount: |
5594 a 4289 c 2181 g 4552 t |
DNA-Sequence: |
NC_001941.txt |
AminoAcid-Sequence: |
NC_001941.txt |
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