|
You are here: Core Forms > Search PCR > PCR
First | Previous | | Next | Last |
PCR |
Name: |
12S-border |
Type: |
Sequencing |
System: |
Sequencing |
Genetic Element: |
12S rRNA (?) |
Ampl.Length: |
340 |
GenomSize: |
|
AnnTemp: |
51 |
Taxonomy: |
Animals |
PCR Description: |
|
Temp. Time Program: |
|
Device: |
|
Master Mix: |
|
nPrimers: |
3 |
Organisation: |
BfR |
Validation: |
NoValidationAvailable |
Sequence: |
NC_001941 |
Remarks: |
Reference Sequence: NC_001941.
PCR-type changed from PCR-RFLP to Sequencing. |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
12S-border-f |
Sense |
gCTAA TACAg TCTAT ATACC gCC |
23 |
59.5 |
50.7 |
66 |
43.5 |
664-686 |
12S-border-r |
Antisense |
TATgC TTACC TTgTT ACgAC TT |
22 |
59.5 |
50.8 |
60 |
36.4 |
1003-982 |
12S-border-Amplicon |
Amplicon |
gctaa tacag tctat atacc gccat cttca gcaaa cccta aaaaa gggac aaaag taagc tcaat aataa cacat aaaga cgtta ggtca aggtg taacc tatgg agtgg gaaga aatgg gctac atttt ctacc caaga aaatt taata cgaaa gccat tatga aatta atagc caaag gagga tttag cagta aacta agaat agagt gctta gttga atcag gccat gaagc acgca cacac cgccc gtcac cctcc tcaag taaat atgat atact taaac ctatt tacat atatc aacca cacga gagga gacaa gtcgt aacaa ggtaa gcata |
340 |
51 |
89.8 |
942 |
38.5 |
664-1003 |
Sequence |
NCBI-Acc: |
NC_001941 |
Sequence Description: |
Ovis aries mitochondrion, complete genome. |
BaseCount: |
5594 a 4289 c 2181 g 4552 t |
DNA-Sequence: |
NC_001941.txt |
AminoAcid-Sequence: |
NC_001941.txt |
|
You are here: Core Forms > Search PCR > PCR © Copyright MolSpec
Disclaimer: MolSpec is not responsible for linked contents.
|