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PCR |
Name: |
Capra-12S |
Type: |
PCR-RFLP |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Genetic Element: |
12S rRNA (?) |
Ampl.Length: |
179 |
GenomSize: |
3.24 |
AnnTemp: |
54 |
Taxonomy: |
Goat |
PCR Description: |
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Temp. Time Program: |
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Device: |
|
Master Mix: |
|
nPrimers: |
3 |
Organisation: |
BfR |
Validation: |
NoValidationAvailable |
Sequence: |
NC_005044 |
Remarks: |
Reference Sequence: NC_005044
Taxon: Only Capra hircus |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Capra-12S-f |
Sense |
TgggC TACAT TTTCT ACCTT AAg |
23 |
62.4 |
53.7 |
64 |
39.1 |
779-801 |
Capra-12S-r |
Antisense |
CgTgT TAATA ggCTT gAgTg C |
21 |
63.2 |
54.4 |
62 |
47.6 |
957-937 |
Capra-12S-Amplicon |
Amplicon |
TGGGC TACAT TTTCT ACCTT AAGaa aatta atacg aaagc catta tgaaa ttaat gacca aagga ggatt tagta gtaaa ctaag aatag agtgc ttagt tgaat taggc catga agcac gcaca caccg cccgt caccc tcctc aagta aatac aatGC ACTCA AGCCT ATTAA CACG |
179 |
54 |
88.3 |
498 |
39.1 |
779-957 |
Sequence |
NCBI-Acc: |
NC_005044 |
Sequence Description: |
Capra hircus mitochondrion, complete genome. |
BaseCount: |
5569 a 4313 c 2189 g 4569 t |
DNA-Sequence: |
NC_005044.txt |
AminoAcid-Sequence: |
NC_005044.txt |
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