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PCR |
Name: |
MY |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Genetic Element: |
Myostatin |
Ampl.Length: |
97 |
GenomSize: |
|
AnnTemp: |
60 |
Taxonomy: |
Animals |
PCR Description: |
|
Temp. Time Program: |
|
Device: |
|
Master Mix: |
|
nPrimers: |
4 |
Organisation: |
BfR |
Validation: |
NoValidationAvailable |
Sequence: |
AB076403 |
Remarks: |
Reference Sequence: AB076403 / AB019620
Publication: Laube, I., Butschke, A., Zagon, J., Spiegelberg, A., Schauzu, M., Bögl, K.-W., Kroh, L. W., Broll, H., 2001. Nachweisverfahren für Rindfleisch in Lebensmitteln unter Anwendung der TaqMan-Technologie. Bundesgesundheitsblatt Gesundheitsforsch. Gesundheitsschutz |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
MY-f |
Sense |
TTgTg CAAAT CCTgA gACTC AT |
22 |
63.8 |
55.1 |
62 |
40.9 |
2480-2501 |
MY-r |
Antisense |
ATACC AgTgC CTggg TTCAT |
20 |
64.8 |
56.0 |
60 |
50.0 |
2576-2557 |
MY-probe |
Marker/Probe |
CCCAT gAAAg ACggT ACAAg gTATA CTg |
28 |
71.4 |
61.9 |
82 |
46.4 |
2506-2533 |
MY-Amplicon |
Amplicon |
TTGTG CAAAT CCTGA GACTC ATcaa aCCCA TGAAA GACGG TACAA GGTAT ACTGg aatcc gatct ctgaa acttg acATG AACCC AGGCA CTGGT AT |
97 |
60 |
87 |
280 |
44.3 |
2480-2576 |
Sequence |
NCBI-Acc: |
AB076403 |
Sequence Description: |
Bos taurus gene for myostatin, complete cds. |
BaseCount: |
2196 a 1116 c 1139 g 2209 t |
DNA-Sequence: |
AB076403.txt |
AminoAcid-Sequence: |
AB076403.txt |
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