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PCR |
Name: |
MY-border |
Type: |
Sequencing |
System: |
Sequencing |
Genetic Element: |
Myostatin |
Ampl.Length: |
327 |
GenomSize: |
|
AnnTemp: |
56 |
Taxonomy: |
Animals |
PCR Description: |
|
Temp. Time Program: |
|
Device: |
|
Master Mix: |
|
nPrimers: |
3 |
Organisation: |
BfR |
Validation: |
NoValidationAvailable |
Sequence: |
AB076403 |
Remarks: |
Reference Sequence: AB076403.
PCR-type changed from PCR-RFLP to Sequencing.
PCRSystem changed from QuaNtitative to Sequencing. |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
MY-border-f |
Sense |
AAACC CAAAT gTTgC TTCTT TAA |
23 |
64.7 |
56.0 |
60 |
30.4 |
2371-2393 |
MY-border-r |
Antisense |
TgggA AggTT ACAgC AAgAT C |
21 |
63.9 |
55.1 |
62 |
47.6 |
2697-2677 |
MY-border-Amplicon |
Amplicon |
AAACC CAAAT GTTGC TTCTT TAAat ttagc tctaa gatac aatac aataa actag taaag gccca actgt ggata tatct gaggc ctgtc aagac tcctg cgaca gtgtT TGTGC AAATC CTGAG ACTCA Tcaaa CCCAT GAAAG ACGGT ACAAG GTATA CTGga atccg atctc tgaaa cttga cATGA ACCCA GGCAC TGGTA Tttgg cagag cattg atgtg aagac agtgt tgcag agctg gctca aacaa cctga atcca actta ggcat tgaaa tcaaa gcttt agatg agaat ggcca tGATC TTGCT GTAAC CTTCC CA |
327 |
56 |
93.2 |
930 |
42.2 |
2371-2697 |
Sequence |
NCBI-Acc: |
AB076403 |
Sequence Description: |
Bos taurus gene for myostatin, complete cds. |
BaseCount: |
2196 a 1116 c 1139 g 2209 t |
DNA-Sequence: |
AB076403.txt |
AminoAcid-Sequence: |
AB076403.txt |
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