Molspec-ID Online Database (MSDB) 1.0 eMail address: Password:   Login

Home Database Overview Taxonomies PCR Primers Sequences Genetic Elements Publications Organisations Persons The Project Project Intro Docs & Publications Slides & Meetings Work Packages Partners Contact

You are here: Core Forms > Search PCR > PCR PCR  Search all Records  First  Previous Entry No. of 31  PCR Name: WP2.2_ELISA-PCR_Myostatin Type: ELISA-PCR System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Genetic Element: Myostatin Ampl.Length: GenomSize: AnnTemp: Taxonomy: Animals PCR Description: WP 2.2: Development of ELISA-PCR based detection and quantification systems. ELISA-PCR system for cattle, pig sheep and horse Target Element: Myostatin Temp. Time Program: PCR profile Taq Activation 95 °C 120 sec Cycles 25 Denat. Amplif. Extension ------------------------------------------------- Target Temp. (°C) 95 62 72 Incubation time (sec) 30 20 30 Final extension 72°C 120 sec Device: Master Mix: nPrimers: Organisation: TIB Validation: Sequence: X56295 Remarks: Target Element: Myostatin Primers PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position MY-r-long Antisense CCAAA TACCA gTgCC TgggT TCA 23 72.1 63.4 70 52.2 2558-2580 BIO_MY-f Sense BIO_T TgTgC AAATC CTgAg ACCAT 21 64.8 55.5 60 42.9 2480-2501 Sequence NCBI-Acc: X56295 Sequence Description: S.scrofa mitochondrion cytb gene for cytochrome b. BaseCount: DNA-Sequence: X56295.txt AminoAcid-Sequence: X56295.txt Publications Short Title Type Date Download Rep: WP2.2_ELISA_PCR_TibMolbiol Report 2004 Rep_WP2.2_ELISA_PCR_TibMolbiol.pdf You are here: Core Forms > Search PCR > PCR © Copyright MolSpec Disclaimer: MolSpec is not responsible for linked contents.