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PCR |
Name: |
Oalb-border |
Type: |
Sequencing |
System: |
Sequencing |
Genetic Element: |
Ovalbumin |
Ampl.Length: |
325 |
GenomSize: |
|
AnnTemp: |
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Taxonomy: |
Birds |
PCR Description: |
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Temp. Time Program: |
|
Device: |
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Master Mix: |
PCR-type changed from PCR-RFLP to Sequencing. |
nPrimers: |
3 |
Organisation: |
BfR |
Validation: |
NoValidationAvailable |
Sequence: |
J00895 |
Remarks: |
Reference Sequence: J00895 M24999 |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
Oalb-border-f |
Sense |
CAATC TgTCT ggCAT CTCCT CA |
22 |
66.1 |
57.3 |
66 |
50 |
8031-8052 |
Oalb-border-r |
Antisense |
CTggA TgCAg CAgAT AACAT ACTTT |
25 |
65.6 |
56.9 |
70 |
40.0 |
8355-8331 |
Oalb-Amplicon |
Amplicon |
CAATC TGTCT GGCAT CTCCT CAgca gagag cctga agata tctca agctg tccat gcagc acatg cagaa atcaa tgaag caggc agaga ggtgg taggg tcagc agagg ctgga gtgga tgctg caagc gtctc tgaag aattt agggc tgacc atcca ttcct cttct gtatc aagca cATCG CAACC AACGC CGTtc tcttc tttgg cagat gtgtt tcccc ttaaa aagaa gaaag ctgaa AAACT CTGTC CCTTC CAACA AGAcc cagag cactg tagta tcagg ggtaa aatga AAAGT ATGTT ATCTG CTGCA TCCA G |
325 |
- |
96 |
960 |
47.7 |
8031-8355 |
Sequence |
NCBI-Acc: |
J00895 M24999 |
Sequence Description: |
Gallus gallus ovalbumin gene, complete cds. |
BaseCount: |
2994 a 1749 c 1721 g 2742 t |
DNA-Sequence: |
J00895.txt |
AminoAcid-Sequence: |
J00895.txt |
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