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You are here: Core Forms > Search PCR > PCR PCR  Search all Records  First  Previous Entry No. of 31  Next  Last  PCR Name: WP2.1QuaL-Multiplex RealTime-PCRCytochromeB Type: Real-Time PCR + Multiplex System: QuaLitative (Light Cycler; TaqMan; Matsunaga) Genetic Element: Mitochondrial Cytochrome b Ampl.Length: 117 GenomSize: AnnTemp: Taxonomy: Animals PCR Description: WP 2.1: Development of multiplex-PCR for species in foods. Qualitative multiplex real time PCR for pig, cattle and myostatin on the Light Cycler, Roche. Target Element: Cytochrome b Temp. Time Program: PCR profile Taq Activation 95 °C 600 sec Cycles 38 Denat. Amplif. Extension ------------------------------------------------- Target Temp. (°C) 95 64 72 Incubation time (sec) 5 5 4 Temp. Transition Rate (°C/sec) 20.0 20.0 20.0 Secondary target temp. (°C) 0 60 0 Step size (°C) 0.0 1.0 0 Step delay (Cycles) 0 10 0 Acquisition mode None Single None Device: Light Cycler, Roche Master Mix: nPrimers: Organisation: TIB Validation: Sequence: X56295 Remarks: Detection of Pig (79) AND Cattle (66) Primers PPName Type PrimerSequence Length T_ann TmThermo Tm2AT %GC Position cons_back_G Antisense gTCTg ATgTg TAgTg TATTg CTAgg AATAg g 31 68.1 59.4 88 41.9 144-174 cons_back_M Antisense gTCgg ATgTg TAgTg TATTg CTAgg AATAg g 31 71.3 62.5 90 45.2 144-174 SIM_F_iFL Sense gACCT CCCAg CTCCA TCAAA CATCT CATCT TgA X T gAA A 38 72.1 72.4 95 44.7 58-95 bos_probe_new Probe_Antisense CCCAg gAggg AACCg AAATT X 20 69.6 60.8 62 55 14607-14627 sus_probe Probe_Antisense CAgAT gCCTA AgAgg gAACC gA X 22 69.3 60.6 68 54.5 98-119 Sequence NCBI-Acc: X56295 Sequence Description: S.scrofa mitochondrion cytb gene for cytochrome b. BaseCount: DNA-Sequence: X56295.txt AminoAcid-Sequence: X56295.txt Publications Short Title Type Date Download Rep: WP2.1_MultiplexPCR_TibMolbiol Report 2004 Rep_WP2.1_MultiplexPCR_TibMolbiol.pdf You are here: Core Forms > Search PCR > PCR © Copyright MolSpec Disclaimer: MolSpec is not responsible for linked contents.