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PCR |
Name: |
PS1 |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
Genetic Element: |
Intergenic spacer region between trnL and trnF exons |
Ampl.Length: |
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GenomSize: |
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AnnTemp: |
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Taxonomy: |
Pea |
PCR Description: |
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Temp. Time Program: |
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Device: |
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Master Mix: |
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nPrimers: |
3 |
Organisation: |
Nestec |
Validation: |
NoValidationAvailable |
Sequence: |
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Remarks: |
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Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
PS1for |
Sense |
CAAAA gCCAA TTAAA TTCCC TCAT |
24 |
66.5 |
57.8 |
64 |
33.3 |
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PS1rev |
Antisense |
gACgC ACCAT CCTAA TTTTA AgAAA |
25 |
66.5 |
57.8 |
68 |
36.0 |
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PS1Pb |
Marker/Probe |
TTgAT ggCCC TTTTT TgATT TAgTT gACAT AgACT C |
36 |
72.1 |
67.1 |
98 |
36.1 |
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