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| PCR |
| Name: |
Sltm |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
| Genetic Element: |
Lectin |
Ampl.Length: |
|
GenomSize: |
|
AnnTemp: |
|
| Taxonomy: |
Soybean |
PCR Description: |
|
Temp. Time Program: |
|
| Device: |
|
Master Mix: |
|
nPrimers: |
3 |
Organisation: |
Nestec |
| Validation: |
NoValidationAvailable |
Sequence: |
K00821 |
| Remarks: |
Reference Sequence: K00821 M30884.
Publication: Vaitilingom M, Pijnenburg H, Gendre F and Brignon P (1999): Real-time quantitative PCR detection of genetically modified maximizer maize and RoundupReady soybean in some representative foods. Journal of Agricultural and Food Chemistry, 47, p. 5261-5266. |
| Primers |
| PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
|
Sltm1 |
Sense |
AACCg gTAgC gTTgC CAg |
18 |
68.5 |
59.7 |
58 |
61.1 |
1253-1270 |
|
Sltm2 |
Antisense |
AgCCC ATCTg CAAgC CTTT |
19 |
68.0 |
59.2 |
58 |
52.6 |
1315-1333 |
|
Sltmp |
Marker/Probe |
TTCgC CgCTT CCTTC AACTT CACCT |
25 |
72.1 |
68.1 |
76 |
52.0 |
1272-1296 |
| Sequence |
| NCBI-Acc: |
K00821 |
Sequence Description: |
Soybean lectin (Le1) gene, complete cds. |
| BaseCount: |
689 a 417 c 341 g 703 t |
| DNA-Sequence: |
K00821.txt |
AminoAcid-Sequence: |
K00821.txt |
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