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| PCR |
| Name: |
UniMit |
Type: |
Real-Time PCR |
System: |
QuaNtitative |
| Genetic Element: |
16S rRNA |
Ampl.Length: |
|
GenomSize: |
|
AnnTemp: |
|
| Taxonomy: |
Universal |
PCR Description: |
|
Temp. Time Program: |
|
| Device: |
|
Master Mix: |
|
nPrimers: |
3 |
Organisation: |
TUGraz |
| Validation: |
NoValidationAvailable |
Sequence: |
|
| Remarks: |
|
| Primers |
| PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
|
UniMit F1 |
Sense |
CTTTg ATAgC ggTTg CACCA T |
21 |
67.4 |
58.6 |
62 |
47.6 |
|
|
UniMit R1 |
Antisense |
CCCIA gggAT AACAg CgCAA TC |
22 |
70.5 |
60.4 |
66 |
54.5 |
|
|
Mit-T |
Marker/Probe |
TCITg ATCCA ACATC gAggT CgTAA ACCC |
29 |
72.1 |
66.3 |
84 |
48.3 |
|
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