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PCR |
Name: |
BosTF |
Type: |
Real-Time PCR |
System: |
QuaLitative (Light Cycler; TaqMan; Matsunaga) |
Genetic Element: |
Transferrin |
Ampl.Length: |
82 |
GenomSize: |
3.7 |
AnnTemp: |
58 |
Taxonomy: |
Cattle |
PCR Description: |
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Temp. Time Program: |
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Device: |
|
Master Mix: |
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nPrimers: |
2 |
Organisation: |
Genescan |
Validation: |
NoValidationAvailable |
Sequence: |
U02564 |
Remarks: |
To do: Calculation of Position & Amplicon |
Primers |
PPName |
Type |
PrimerSequence |
Length |
T_ann |
TmThermo |
Tm2AT |
%GC |
Position |
BosTF |
Sense |
CAAgA ATgCT ACTTg gCAAT gg |
22 |
66.7 |
57.9 |
64 |
45.5 |
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BosTF |
Antisense |
gTTCC CAgAT CACAT CCTCC TT |
22 |
65.9 |
57.2 |
66 |
50.0 |
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Sequence |
NCBI-Acc: |
U02564 |
Sequence Description: |
Bos taurus transferrin mRNA, complete cds. |
BaseCount: |
667 a 514 c 613 g 544 t |
DNA-Sequence: |
U02564.txt |
AminoAcid-Sequence: |
U02564.txt |
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